Molecular Formula | C16H15F5N2O3S |
Molar Mass | 410.36 |
Density | 1.441 |
Melting Point | 190.0 to 194.0 °C |
Boling Point | 555.3±50.0 °C(Predicted) |
pKa | 13.89±0.40(Predicted) |
Storage Condition | Keep in dark place,Inert atmosphere,Store in freezer, under -20°C |
overview | chemical modifications of biomolecules are well known in the art. Reagents and methods for labeling, labeling and reporting covalent linkage of molecules to biomolecules provide valuable tools in the fields of biochemistry, molecular biology and diagnostics. Labeled biomolecules are particularly useful for detecting target components and separating target molecules in biological samples. Utilize specific binding entities, including biotin-avidin/streptavidin system. Biomolecules are preferably linked to biotin, and avidin (or streptavidin) conjugated fluorescent dyes or enzymes are used to detect biotin labeling. |
Application | The specific binding affinity between biotin and avidin (or streptavidin) conjugate provides the specificity of attaching fluorescent dyes, enzymes or EM markers to biomolecules. Biotin ester of pentafluorophenol is a chemical modification product of biotin, biotin of pentafluorophenol was synthesized by pentafluorophenol and D-biotin in a yield of about 98%. Biotin of pentafluorophenol was synthesized by pentafluorophenyl trifluoroacetate and D-biotin in a yield of about 95%. |